Order NextCollectTM Kit

Urine Collection and DNA Preservation Kit

NextCollect is a first of its kind proprietary high volume urine specimen collection and stabilization kit.

Formulated DNA preservative solution is integrated into the NextCollect reservoir cap, and dispensed when secured to the NextCollect cup. When added to the urine specimen, NextCollect preserves DNA during transport for up to 2 weeks at room temperature.

NextCollect is designed to collect a higher volume of urine specimen, containing more DNA available for testing methods. NextCollect urine extracted DNA can be used for a range of applications across oncology, urology, virology and infectious disease.

NextCollect is manufactured for Research Use Only and not cleared for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.


First ever automated DNA stabilizing device for urine collection


No specimen transfer,
easy to use 2-step collection


Collects and preserves transrenal
cfDNA (systemic), urinary tract
cfDNA, and germline DNA

Thank you for your interest in NextCollect. We are currently engaging large business to business and technology transfer opportunities. Please contact us at NextCollect@trovagene.com.

Urine Collection Kit

Materials Provided

Each kit contains:

Package Insert with Collection Instructions
Urine Collection Cup

Plastic cup for 200mL (cc) urine specimen collection and transport

Cap with Preservative Reservoir
Specimen Patient Identification Label
Absorbent Sheet
Biohazard Bag

See reverse side for sample collection instructions.

Kit Storage

Store the Urine Collection Kit at room temperature (15°C to 30°C/59°F to 86°F).

Sample Storage and Transport

Preserved urine specimen is stable at room temperature, between 15°C to 30°C /59°F to 86°F, for up to 14 days.

Warnings and Precautions

Avoid puncturing the preservative film inside the cap prior to securing the cap on the urine collection cup.
If preservative solution contacts skin, rinse thoroughly
with water.

How It Works

Remove urine collection cup from kit. Leave cap affixed to box lid.

Complete specimen patient identification label with patient's name, date of birth(DOB) and date of collection(DOC) and affix label to urine collection cup.

Collect 200mL(cc) of urine into urine collection cup. Fill between minimum and maximum lines indicated on the cup.

Immediately after filling urine collection cup, remove cap from kit and secure onto cup.

Secure cap tightly on to cup, twisting past the audible "click" indicating the cap is completely fastened to the cup.

Observe preservative reagent solution releasing into urine sample. If preservative reagent does not release, untwist cap 1/4 turn and re-twist the cap onto the cup to ensure closure. Once securely fastened, gently swirl cup to mix preservative with the urine.

Place the urine collection cup in biohazard bag and seal bag to close.

Place sealed biohazard bag inside shipping box.

Ship as soon as possible.


What volume of urine will NextCollect support?

Up to 200ml of urine can be collected in the cup, the preservative is another 30ml when released, and NextCollect total capacity is 250ml.

How much urine is in a normal void?

On average a typical urination provides 150-300ml, however, results can vary depending on the time of day, level of hydration, and treatment of illness.

Does the user need to drink a lot of water prior to urine sample collection?

No, the user can drink the same amount that they would normally drink. Drinking an excessive amount of water simply dilutes the sample.

How will the user know when NextCollect is sealed?

Twist the cap onto the cup firmly, and there is an audible “click” indicating the cap is fully sealed onto the cup. The plastic film holding the preservative into the cap will make a tearing or puncturing sound when you twist, so the user will need to twist all the way through to the secure “click” lock for the cup to be sealed.

What happens if the user twists the NextCollect reservoir cap on to the cup, and the urine preservative solution does not release?

First, check to be sure the threads of the cap are aligned with the threads of the cup, and the cap isn’t tilted or offset. Untwist the cap off and try again. If the cap is on correctly, make sure to tighten all the way to the audible “click” indicating the cap is secure. If solution is still not released, loosen cap 1/4 to 1/2 turn without completely removing, and re-tighten.

If the user has trouble filling the cup in one urination, can they collect multiple urinations over time?

Yes, if it takes multiple urinations to provide adequate volume that is ok. For the first urination, secure the cap to the cup to release the preservative solution. Re-open the cup to add subsequent urinations, re-fasten tightly all the way to the audible “click”, then prepare for shipment.

What is the minimum amount of urine needed?

This varies depending on the test being done. Some tests require only a few milliliters; others require hundreds of milliliters. In general, it is best to try to reach the 200ml fill line unless the test provider has indicated a specific target fill volume for your test.

How stable is the urine once the cap is secured?

After ensuring the cap is tight, the device and specimen are very secure. NextCollect is manufactured with strong materials to withstand bumps and pressure changes during transport. DNA stability of the preserved specimen has been tested for up to 2 weeks at room temperature.

Can NextCollect be centrifuged?

Yes, NextCollect was designed to be placed directly into a centrifuge.

Can NextCollect be used with other available DNA extraction kits or methods?

NextCollect has been tested with a variety of available DNA extraction kits, and choosing the correct kit will depend on the downstream assay being performed.

If I intend to bank the sample, is it better to extract or isolate the DNA sample from NextCollect right away or freeze the entire urine specimen?

The preservative inside NextCollect will preserve all nucleic acid at –80°C for an extended period of time, however, a freeze/thaw cycle will lyse cells in the specimen releasing their genomic DNA contents into the sample and this should be factored into the test being performed. It also is possible to spin down the specimen directly in the NextCollect device, and then store the supernatant and pellets separately if cellular DNA is a contaminant for your specific downstream assay.

How do I dispose of a used urine cup?

Dispose of the collection cup in a bio-hazard bag.

Product Documents

Trovagene NextCollect US MSDS

Product Insert

Have More Questions?

To learn more about NextCollectTM contact us at NextCollect@trovagene.com